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Effect of excess water on the desilylation of oligoribonucleotides using tetrabutylammonium fluoride.

The most commonly available 2' hydroxyl protecting group used in the synthesis of oligoribonucleotides is the tert-butyldimethylsilyl moiety. This protecting group is generally cleaved with 1 M tetrabutylammonium fluoride (TBAF) in tetrahydrofuran (THF). The efficiency of this reaction was test...

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Hlavní autoři: Hogrefe, R I, McCaffrey, A P, Borozdina, L U, McCampbell, E S, Vaghefi, M M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1993
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC331499/
https://ncbi.nlm.nih.gov/pubmed/8233822
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