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Mapping of contact sites in complex formation between light-activated rhodopsin and transducin by covalent crosslinking: Use of a chemically preactivated reagent

Contact sites in interaction between light-activated rhodopsin and transducin (T) have been investigated by using a chemically preactivated crosslinking reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The 3 propionyl-N-succinimidyl group in the reagent was attached by a disulfide exchange rea...

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Detalhes bibliográficos
Main Authors: Itoh, Yoshiki, Cai, Kewen, Khorana, H. Gobind
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC33132/
https://ncbi.nlm.nih.gov/pubmed/11320238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051632998
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