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Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: Use of a photoactivatable reagent

Interaction of light-activated rhodopsin with transducin (T) is the first event in visual signal transduction. We use covalent crosslinking approaches to map the contact sites in interaction between the two proteins. Here we use a photoactivatable reagent, N-[(2-pyridyldithio)-ethyl], 4-azido salicy...

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Detalhes bibliográficos
Main Authors: Cai, Kewen, Itoh, Yoshiki, Khorana, H. Gobind
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC33131/
https://ncbi.nlm.nih.gov/pubmed/11320237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051632898
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