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Stability of single-nucleotide bulge loops embedded in a GAAA RNA hairpin stem

Forty-six RNA hairpins containing combinations of 3′ or 5′ bulge loops and a 3′ or 5′ fluorescein label were optically melted in 1 M NaCl, and the thermodynamic parameters ΔH°, ΔS°, ΔG°(37), and T(M) for each hairpin were determined. The bulge loops were of the group I variety, in which the identity...

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Detalhes bibliográficos
Main Authors: Lim, Geoffrey F.S., Merz, Gregory E., McCann, Michael D., Gruskiewicz, Jocelyn M., Serra, Martin J.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3312567/
https://ncbi.nlm.nih.gov/pubmed/22345128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.028308.111
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