Measurements of single DNA molecule packaging dynamics in bacteriophage λ reveal high forces, high motor processivity, and capsid transformations

Molecular motors drive genome packaging into preformed procapsids in many dsDNA viruses. Here, we present optical tweezers measurements of single DNA molecule packaging in bacteriophage λ. DNA-gpA-gpNu1 complexes were assembled with recombinant gpA and gpNu1 proteins and tethered to microspheres, an...

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Detalhes bibliográficos
Main Authors: Fuller, Derek N., Raymer, Dorian M., Rickgauer, John Peter, Robertson, Rae M., Catalano, Carlos E., Anderson, Dwight L., Grimes, Shelley, Smith, Douglas E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3311920/
https://ncbi.nlm.nih.gov/pubmed/17919653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.09.011
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