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Separation of nucleobases and their derivatives with organic-high ionic strength aqueous phase systems by spiral high-speed counter-current chromatography

A set of nucleic acid constituents were separated with ultra polar two-phase solvent systems by a spiral multilayer coil mounted on the rotary frame of a type-J coil planet centrifuge. These two-phase systems were composed of 1-butanol/ethanol/50% saturated aqueous ammonium sulfate at various volume...

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Detalhes bibliográficos
Main Authors: Shibusawa, Yoichi, Yanagida, Akio, Ogihara, Atsushi, Ma, Ying, Chen, Xiaoyuan, Ito, Yoichiro
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3311770/
https://ncbi.nlm.nih.gov/pubmed/22391329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2012.02.012
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