Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein

The gene encoding the L1 ribosomal protein from Burkholderia pseudomallei strain D286 has been cloned into the pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. Crystals of the native protein were grown by the hanging-drop vapour-diffusion technique using PEG 3350 as a precipi...

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Bibliografske podrobnosti
Main Authors: Abd Aziz, Abd Ghani, Ruzheinikov, Sergey N., Sedelnikova, Svetlana E., Mohamed, Rahmah, Nathan, Sheila, Baker, Patrick J., Rice, David W.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2012
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3310549/
https://ncbi.nlm.nih.gov/pubmed/22442241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112004800
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