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Expression, purification, crystallization and preliminary X-ray diffraction analysis of a galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum

A galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the tetragonal space group P4(1), with unit-cell parameter...

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Detalhes bibliográficos
Main Authors: Satomura, Takenori, Hiraki, Akihiro, Kawai, Tomoyuki, Kawakami, Ryushi, Ohshima, Toshihisa, Sakuraba, Haruhiko
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3310544/
https://ncbi.nlm.nih.gov/pubmed/22442236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112003880
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