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Identifying microbial fitness determinants by Insertion Sequencing (INSeq) using genome-wide transposon mutant libraries
Insertion Sequencing (INSeq) is a method for determining the insertion site and relative abundance of large numbers of transposon mutants in a mixed population of isogenic mutants of a sequenced microbial species. INSeq is based on a modified mariner transposon containing MmeI sites at its ends, all...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3310428/ https://ncbi.nlm.nih.gov/pubmed/22094732 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.417 |
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