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Amplification of plant U3 and U6 snRNA gene sequences using primers specific for an upstream promoter element and conserved intragenic regions.

U-snRNA genes in higher plants contain two essential promoter elements, the USE with sequence RTCCCACATCG and the TATA-like box, positioned in the -70 and -30 regions, respectively. Using an oligodeoxynucleotide containing the USE motif and oligodeoxynucleotides specific for the intragenic regions c...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Marshallsay, C, Kiss, T, Filipowicz, W
Format: Artigo
Language:Inglês
Published: Oxford University Press 1990
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330997/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2362803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.12.3459
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