Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants.
A saturating oligonucleotide-directed mutagenesis of both tet operators in the tet regulatory sequence was performed yielding mutants with four identical base pair exchanges at equivalent positions in the four tet operator half sides. The mutants were cloned between bipolar lacZ and galK indicator g...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1990
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330813/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2161514/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.10.2875 |
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