A computer program for selection of oligonucleotide primers for polymerase chain reactions.

We have designed a computer program which rapidly scans nucleic acid sequences to select all possible pairs of oligonucleotides suitable for use as primers to direct efficient DNA amplification by the polymerase chain reaction. This program is based on a set of rules which define in generic terms bo...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Lowe, T, Sharefkin, J, Yang, S Q, Dieffenbach, C W
Format: Artigo
Language:Inglês
Published: Oxford University Press 1990
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330593/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1692404/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.7.1757
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