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Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10 nm lateral resolution and <20 nm axial resolution when imaging biological specimens. Using this approach, we resolved individ...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3304438/ https://ncbi.nlm.nih.gov/pubmed/22231642 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1841 |
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