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Insertional gene synthesis, a novel method of assembling consecutive DNA sequences within specific sites in plasmids. Construction of the HIV-1 tat gene.

The construction of the HIV-1 tat gene using a novel method termed insertional gene synthesis (IGS) is described. IGS is used to assemble a gene or any DNA sequence in a stepwise manner within a plasmid containing a single stranded DNA phage origin of replication. The IGS method is based upon consec...

詳細記述

保存先:
書誌詳細
出版年:Nucleic Acids Res
主要な著者: Ciccarelli, R B, Loomis, L A, McCoon, P E, Holzschu, D L
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1990
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330440/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2157195/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.5.1243
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