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Selection of Diethylstilbestrol-Specific Single-Chain Antibodies from a Non-Immunized Mouse Ribosome Display Library

Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating...

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Hlavní autoři: Sun, Yanan, Ning, Baoan, Liu, Ming, Gao, Xianjun, Fan, Xianjun, Liu, Jianqing, Gao, Zhixian
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3302827/
https://ncbi.nlm.nih.gov/pubmed/22427984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0033186
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