In vitro suppression of an amber mutation by a chemically aminoacylated transfer RNA prepared by runoff transcription.

An amber suppressor tRNA was prepared in vitro by runoff transcription with T7 RNA polymerase. Both full-length tRNA and truncated tRNA lacking the 3' terminal pCpA from the acceptor stem could be synthesized from the same DNA template. Truncated runoff suppressor tRNA could be enzymatically ligated...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Noren, C J, Anthony-Cahill, S J, Suich, D J, Noren, K A, Griffith, M C, Schultz, P G
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1990
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330206/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2308838/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.1.83
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