Development of a Markerless Gene Replacement System for Acidithiobacillus ferrooxidans and Construction of a pfkB Mutant

The extremely acidophilic, chemolithoautotrophic Acidithiobacillus ferrooxidans is an important bioleaching bacterium of great value in the metallurgical industry and environmental protection. In this report, a mutagenesis system based on the homing endonuclease I-SceI was developed to produce targe...

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Hlavní autoři: Wang, Huiyan, Liu, Xiangmei, Liu, Shuangshuang, Yu, Yangyang, Lin, Jianqun, Lin, Jianqiang, Pang, Xin, Zhao, Jian
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2012
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3298148/
https://ncbi.nlm.nih.gov/pubmed/22210219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07230-11
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