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Serial two-photon tomography: an automated method for ex-vivo mouse brain imaging

Here we describe an automated method, which we call serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and ca...

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Bibliografiske detaljer
Main Authors: Ragan, Timothy, Kadiri, Lolahon R., Venkataraju, Kannan Umadevi, Bahlmann, Karsten, Sutin, Jason, Taranda, Julian, Arganda-Carreras, Ignacio, Kim, Yongsoo, Seung, H. Sebastian, Osten, Pavel
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3297424/
https://ncbi.nlm.nih.gov/pubmed/22245809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1854
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