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Oocyte formation by mitotically-active germ cells purified from ovaries of reproductive age women
Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries. Here we describe and validate a FACS-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mit...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3296965/ https://ncbi.nlm.nih.gov/pubmed/22366948 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nm.2669 |
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