Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection

INTRODUCTION: Accurate analyses of microbiota composition of low-density communities (10(3)–10(4) bacteria/sample) can be challenging. Background DNA from chemicals and consumables, extraction biases as well as differences in PCR efficiency can significantly interfere with microbiota assessment. Thi...

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Bibliographische Detailangaben
Hauptverfasser: Biesbroek, Giske, Sanders, Elisabeth A. M., Roeselers, Guus, Wang, Xinhui, Caspers, Martien P. M., Trzciński, Krzysztof, Bogaert, Debby, Keijser, Bart J. F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2012
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3295791/
https://ncbi.nlm.nih.gov/pubmed/22412957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032942
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