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Simultaneous Detection of Infectious Human Echoviruses and Adenoviruses by an In Situ Nuclease-Resistant Molecular Beacon-Based Assay

A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible w...

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Detalhes bibliográficos
Main Authors: Dunams, Daniela, Sarkar, Payal, Chen, Wilfred, Yates, Marylynn V.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3294457/
https://ncbi.nlm.nih.gov/pubmed/22194298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05937-11
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