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BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177.

A new type II restriction endonuclease designated BsiY I has been purified from a thermophilic soil Bacillus stearothermophilus strain. This enzyme recognizes and cleaves the highly degenerate sequence 5' CCNNNNN!NNGG 3'. During the identification of the recognition sequence of BsiY I, we discovered...

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Udgivet i:Nucleic Acids Res
Principais autores: Mok, Y K, Clark, D R, Kam, K M, Shaw, P C
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1991
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC329437/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2041772/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.9.2321
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