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Catalytic contributions of key residues in the adenine glycosylase MutY revealed by pH dependent kinetics and cellular repair assays

MutY enzymes prevent mutations in DNA associated with 8-oxoguanine (OG) by catalyzing the removal of adenines opposite OG. pH dependence analyses of the adenine glycosylase activity establish that Asp 138 of MutY must be deprotonated for maximal catalytic activity consistent with the role of this re...

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Bibliographic Details
Main Authors: Brinkmeyer, Megan K., Pope, Mary Ann Miles, David, Sheila S.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3292766/
https://ncbi.nlm.nih.gov/pubmed/22365610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2011.11.011
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