Feeder Cells Support the Culture of Induced Pluripotent Stem Cells Even after Chemical Fixation

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cel...

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Detalhes bibliográficos
Main Authors: Yue, Xiao-Shan, Fujishiro, Masako, Nishioka, Chieko, Arai, Takashi, Takahashi, Eiki, Gong, Jian-Sheng, Akaike, Toshihiro, Ito, Yoshihiro
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3292564/
https://ncbi.nlm.nih.gov/pubmed/22396791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0032707
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