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A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.
We describe the use of a Xenopus laevis egg extract for the in vitro translation and post translational modification of membrane and secretory proteins. This extract is capable of the translation and segregation into membranes of microgram per millilitre levels of protein from added mRNAs. Signal se...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1991
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC329185/ https://ncbi.nlm.nih.gov/pubmed/1754376 |
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