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The inhibition of restriction endonuclease PvuII cleavage activity by methylation outside its recognition sequence.
The recombinant plasmid pGEM4Z-ras DNA which was methylated on dam and dcm sites outside the PvuII recognition sequence was digested with restriction endonuclease PvuII, and one of the three PvuII sites was about 16-fold less efficient to cleave than either of the other two. On the contrary, the thr...
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| Main Authors: | , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1991
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328978/ https://ncbi.nlm.nih.gov/pubmed/1945846 |
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