Evaluation of DNA Extraction Methods and Their Clinical Application for Direct Detection of Causative Bacteria in Continuous Ambulatory Peritoneal Dialysis Culture Fluids from Patients with Peritonitis by Using Broad-Range PCR

BACKGROUND: The aims of this study were to compare several DNA extraction methods and 16S rDNA primers and to evaluate the clinical utility of broad-range PCR in continuous ambulatory peritoneal dialysis (CAPD) culture fluids. METHODS: Six type strains were used as model organisms in dilutions from...

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Detalhes bibliográficos
Main Authors: Kim, Si Hyun, Jeong, Haeng Soon, Kim, Yeong Hoon, Song, Sae Am, Lee, Ja Young, Oh, Seung Hwan, Kim, Hye Ran, Lee, Jeong Nyeo, Kho, Weon-Gyu, Shin, Jeong Hwan
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society for Laboratory Medicine 2012
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3289776/
https://ncbi.nlm.nih.gov/pubmed/22389878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3343/alm.2012.32.2.119
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