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A Temperature-Dependent Conformational Change of NADH Oxidase from Thermus thermophilus HB8

Using molecular dynamics simulations and steady-state fluorescence spectroscopy, we have identified a conformational change in the active site of a thermophilic flavoenzyme, NADH oxidase from Thermus thermophilus HB8 (NOX). The enzyme’s far-UV circular dichroism spectrum, intrinsic tryptophan fluore...

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Main Authors: Merkley, Eric D., Daggett, Valerie, Parson, William W.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3288523/
https://ncbi.nlm.nih.gov/pubmed/22081476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.23219
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