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Direct introduction and transient expression of capped and non-capped RNA in Saccharomyces cerevisiae.

We report the introduction of functional RNA molecules into yeast spheroplasts. Plasmids containing the firefly luciferase coding region were transcribed to yield RNAs suitable for introduction into yeast cells and direct assay of their translation products. The 5' noncoding regions of the RNAs...

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Détails bibliographiques
Auteurs principaux: Russell, P J, Hambidge, S J, Kirkegaard, K
Format: Artigo
Langue:Inglês
Publié: 1991
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC328795/
https://ncbi.nlm.nih.gov/pubmed/1656383
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