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Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast.

Yeast artificial chromosomes (YACs) provide a powerful tool for the isolation and mapping of large regions of mammalian chromosomes. We developed a rapid and efficient method for the isolation of DNA fragments representing the extreme ends of YAC clones by the insertion of a rescue plasmid into the...

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Détails bibliographiques
Auteurs principaux: Hermanson, G G, Hoekstra, M F, McElligott, D L, Evans, G A
Format: Artigo
Langue:Inglês
Publié: 1991
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC328794/
https://ncbi.nlm.nih.gov/pubmed/1923762
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