Systematic exploration of essential yeast gene function with temperature-sensitive mutants

Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (~45%) of the 1,101 essential yeast genes, with ~30% of the genes represented by multiple alleles. All of the alleles...

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Detalhes bibliográficos
Main Authors: Li, Zhijian, Vizeacoumar, Franco J, Bahr, Sondra, Li, Jingjing, Warringer, Jonas, Vizeacoumar, Frederick S, Min, Renqiang, VanderSluis, Benjamin, Bellay, Jeremy, DeVit, Michael, Fleming, James A, Stephens, Andrew, Haase, Julian, Lin, Zhen-Yuan, Baryshnikova, Anastasia, Lu, Hong, Yan, Zhun, Jin, Ke, Barker, Sarah, Datti, Alessandro, Giaever, Guri, Nislow, Corey, Bulawa, Chris, Myers, Chad L, Costanzo, Michael, Gingras, Anne-Claude, Zhang, Zhaolei, Blomberg, Anders, Bloom, Kerry, Andrews, Brenda, Boone, Charles
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3286520/
https://ncbi.nlm.nih.gov/pubmed/21441928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1832
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