Polymerase chain reaction mapping of yeast GAL7 mRNA polyadenylation sites demonstrates that 3' end processing in vitro faithfully reproduces the 3' ends observed in vivo.
In general, synthetic RNA transcripts corresponding to the 3' ends of Saccharomyces cerevisiae genes appear to be accurately cleaved and polyadenylated in vitro under appropriate conditions in yeast cell extracts. Initially, however, the endpoints observed in vitro for the GAL7 gene failed to correl...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1991
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| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328398/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1677180/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.13.3683 |
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