Termination of transcription in an 'in vitro' system is dependent on a polyadenylation sequence.
Using HeLa cell nuclear extract as a source of the different transcription and polyadenylation factors and reverse transcription to analyze the levels of RNA 5' and 3' to the cleavage-polyadenylation site, an in vitro assay has been established to study polyadenylation coupled to transcription direc...
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| Veröffentlicht in: | Nucleic Acids Res |
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| 1. Verfasser: | |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1991
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328384/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1712938/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.13.3593 |
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