Attenuation of Vaccinia Tian Tan Strain by Removal of Viral TC7L-TK2L and TA35R Genes

Vaccinia Tian Tan (VTT) was attenuated by deletion of the TC7L-TK2L and TA35R genes to generate MVTT3. The mutant was generated by replacing the open reading frames by a gene encoding enhanced green fluorescent protein (EGFP) flanked by loxP sites. Viruses expressing EGFP were then screened for and...

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Bibliografiske detaljer
Main Authors: Kan, Shifu, Wang, Yuhang, Sun, Lili, Jia, Peng, Qi, Yanxin, Su, Jiaqiang, Liu, Lei, Yang, Guohua, Liu, Liming, Wang, Zhuoyue, Wang, Jinhui, Liu, Guangchen, Jin, Ningyi, Li, Xiao, Ding, Zhuang
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3283712/
https://ncbi.nlm.nih.gov/pubmed/22363781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0031979
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