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In situ hybridization with fluoresceinated DNA.
We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection s...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1991
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328316/ https://ncbi.nlm.nih.gov/pubmed/2062640 |
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