The use of BrCN for assembling modified DNA duplexes and DNA-RNA hybrids; comparison with water-soluble carbodiimide.

Both cyanogen bromide (BrCN) and 1-ethyl-3-(3'-dimethylaminopropyl) carbodiimide may be used as coupling reagents for the template-directed assembly of DNA duplexes containing the sugar-phosphate backbone modification. Both reagents show similar ligation site structure-specific trend. Practical reco...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Dolinnaya, N G, Sokolova, N I, Ashirbekova, D T, Shabarova, Z A
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1991
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328272/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1711679/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.11.3067
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