Determination of the Proton Environment of High Stability Menasemiquinone Intermediate in Escherichia coli Nitrate Reductase A by Pulsed EPR

Escherichia coli nitrate reductase A (NarGHI) is a membrane-bound enzyme that couples quinol oxidation at a periplasmically oriented Q-site (Q(D)) to proton release into the periplasm during anaerobic respiration. To elucidate the molecular mechanism underlying such a coupling, endogenous menasemiqu...

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Detalhes bibliográficos
Main Authors: Grimaldi, Stéphane, Arias-Cartin, Rodrigo, Lanciano, Pascal, Lyubenova, Sevdalina, Szenes, Rodolphe, Endeward, Burkhard, Prisner, Thomas F., Guigliarelli, Bruno, Magalon, Axel
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Bioenergetics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3281603/
https://ncbi.nlm.nih.gov/pubmed/22190684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.325100
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