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A simple method for quantifying specific mRNAs in small numbers of early mouse embryos.

Amplification of sequences by the polymerase chain reaction (PCR) has become a powerful tool in the study of gene expression. The technique is, in fact, so powerful that it may detect 'leaky transcription'. Thus, it is now important to be able to quantify the transcripts that are amplified...

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Detalhes bibliográficos
Main Authors: Gaudette, M F, Crain, W R
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC328118/
https://ncbi.nlm.nih.gov/pubmed/1903203
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