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Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP)
In the “GFP reconstitution across synaptic partners” (GRASP) method, non-fluorescent fragments of GFP are expressed in two different neurons; the fragments self-assemble at synapses between the two to form a fluorophore. GRASP has proven useful for light microscopic identification of synapses in two...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Frontiers Media S.A.
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3280602/ https://ncbi.nlm.nih.gov/pubmed/22355283 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnmol.2012.00018 |
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