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Cloning of chicken embryo tRNA genes using single stranded nucleosomal DNA highly enriched for tRNA complementary sequences

DNA from chicken embryo nucleosome tetramers (about 760 base pairs in size) was enriched for tRNA genes by RPC-5 chromatography. The enriched DNA was hybridized with chicken embryo total tRNA and the hybridized DNA isolated utilizing a) avidinbiotin interaction, b) diazobenzyloxymethyl paper, and c)...

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Detalles Bibliográficos
Main Authors: Wittig, Burghardt, Wittig, Stephanie, Grunz, Horst
Formato: Artigo
Idioma:Inglês
Publicado: 1979
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC327976/
https://ncbi.nlm.nih.gov/pubmed/493122
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