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Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313.

By employing a system of RNA polymerase binding and restriction endonuclease digestion, we demonstrate that the region in and around the Hind III site of pBR313 and pBR322 is the promoter for the tetracycline (Tc) resistance gene(s). Furthermore, it is shown that this region was transferred intact f...

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Autores principales: Rodriguez, R L, West, R W, Heyneker, H L, Bolivar, F, Boyer, H W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1979
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC327933/
https://ncbi.nlm.nih.gov/pubmed/384366
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