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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Several methods for the preparation of murine dendritic cells can be found in the literature. Here, we present a method that produces greater than 85% CD11c high dendritic cells in culture that home to the draining lymph node after subcutaneous injection and present antigen to antigen specific T cel...

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Библиографические подробности
Главные авторы: P. Matheu, Melanie, Sen, Debasish, Cahalan, Michael D, Parker, Ian
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2008
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3278326/
https://ncbi.nlm.nih.gov/pubmed/19066518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/773
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