Fluorescence Detection of MMP-9. I. MMP-9 Selectively Cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide

MMP-9 enzyme recognizes a peptide sequence Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys and cleaves the peptide into two parts. We synthesized a dual fluorophore beacon consisting of 5-FAM and Cy5 dyes. The fluorescence emission of the fluorescein moiety is dramatically quenched by Cy5 molecule due to Förste...

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Hauptverfasser: Fudala, Rafal, Ranjan, Amalendu P., Mukerjee, Anindita, Vishwanatha, Jamboor K., Gryczynski, Zygmunt, Borejdo, Julian, Sarkar, Pabak, Gryczynski, Ignacy
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277814/
https://ncbi.nlm.nih.gov/pubmed/21446907
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