Extending the Dynamic Range of Label-free Mass Spectrometric Quantification of Affinity Purifications

Affinity purification (AP) of protein complexes combined with LC-MS/MS analysis is the current method of choice for identification of protein-protein interactions. Their interpretation with respect to significance, specificity, and selectivity requires quantification methods coping with enrichment f...

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Main Authors: Bildl, Wolfgang, Haupt, Alexander, Müller, Catrin S., Biniossek, Martin L., Thumfart, Jörg Oliver, Hüber, Björn, Fakler, Bernd, Schulte, Uwe
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2012
Fag:
Technical Innovation and Resources
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277747/
https://ncbi.nlm.nih.gov/pubmed/22067099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.007955
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