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Global kinetic analysis of proteolysis via quantitative targeted proteomics

Mass spectrometry-based proteomics is a powerful tool for identifying hundreds to thousands of posttranslational modifications in complex mixtures. However, it remains enormously challenging to simultaneously assess the intrinsic catalytic efficiencies (k(cat)/K(M)) of these modifications in the con...

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Détails bibliographiques
Auteurs principaux: Agard, Nicholas J., Mahrus, Sami, Trinidad, Jonathan C., Lynn, Aenoch, Burlingame, Alma L., Wells, James A.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3277568/
https://ncbi.nlm.nih.gov/pubmed/22308409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1117158109
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