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Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.
Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli wa...
Tallennettuna:
Päätekijät: | , , , |
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Aineistotyyppi: | Artigo |
Kieli: | Inglês |
Julkaistu: |
1979
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Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327742/ https://ncbi.nlm.nih.gov/pubmed/375200 |
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