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Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.
Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli wa...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1979
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327742/ https://ncbi.nlm.nih.gov/pubmed/375200 |
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