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Ultraviolet light-induced crosslinking of mRNA to proteins.
Irradiation of intact or EDTA-dissociated L-cell polyribosomes with 254 nm UV light at doses of 1-2 x 10(5) ergs/mm2 extensively crosslinks mRNA to proteins. The crosslinked mRNA-protein complexes can be isolated on the basis of buoyant density in urea-containing CS2SO4 gradients that dissociate non...
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| Glavni autor: | |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1979
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| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327723/ https://ncbi.nlm.nih.gov/pubmed/424311 |
| Oznake: |
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