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Protein-binding dynamics imaged in a living cell
Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated b...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3277120/ https://ncbi.nlm.nih.gov/pubmed/22307600 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1112171109 |
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