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Purification and characterization of a uracil-DNA glycosylase from the yeast. Saccharomyces cerevisiae.

An activity which releases free uracil from bacteriophage PBS1 DNA has been purified over 10,000 fold from extracts of Saccharomyces cerevisiae. The enzyme is active on both native and denatured PBS1 DNA and is active in the absence of divalent cation, and in the presence of 1 mM EDTA. The enzyme ha...

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Detalhes bibliográficos
Main Authors: Crosby, B, Prakash, L, Davis, H, Hinkle, D C
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC327561/
https://ncbi.nlm.nih.gov/pubmed/7031606
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